High-Level Expression of Recombinant VHSV Glycoprotein Using Transformed C. vulgaris and Verification of Vaccine Efficacy
نویسندگان
چکیده
The demand for aquaculture is increasing, but production declining due to high feed costs and disease outbreaks. Viral hemorrhagic septicemia (VHS) a viral that seriously affects seawater freshwater fish in aquaculture, including the olive flounder (Paralichthys olivaceus), major Korea. However, very few vaccines are currently available virus (VHSV). nutrient-rich microalga Chlorella vulgaris has been used as additive host industrial of recombinant VHSV glycoprotein an oral vaccine. In this study, was cloned with salt-inducible promoter, levels expression up 41.1 mg/g wet C. vulgaris, representing 27.4% total extracted soluble protein, were achieved by growing transformed 5 days presence 250 mM NaCl. neutralizing antibody detected serum given containing 9% glycoprotein-expressing vulgaris. Furthermore, relative survival rates 100% 81.9% following challenges these at 106 107 pfu/fish, respectively, indicating could be platform proteins use control diseases aquaculture.
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ژورنال
عنوان ژورنال: Vaccines
سال: 2023
ISSN: ['2076-393X']
DOI: https://doi.org/10.3390/vaccines11071205